BS EN 1390:2020 pdf download.Wood preservatives - Determination of the eradicant action against Hylotrupes bajulus(Linnaeus) larvae — Laboratory method.
5 Test materials
5.1 BIological material
5.1.1 HyloIrupes bajulus (Llnnaeus) larvae
5.1.2 Source of larvae
The larvae shall preferably be obtained from cultures reared according to the method described in Annex B.
Larvae can also be taken from naturally infested wood. in which case they should be transferred into sapwood of pine and stored for at least four weeks under the rearing conditions specified in Annex B.
5.1.3 Provision of larvae
Carefully split or crumble InEestt!d blocks to extract larvae.
Keep the larvae separate from one another in glass receptacles for two or three days in the culturing chamber (5.3.1) to check they are healthy.
5.1.4 ChoIce of larvae
Use only healthy larvae in the test.
NOTE A healthy larva can be recognized by Ivoly-white colour. Its firm consistency and rounded appearance, and by the absence of wounds or bites. whrch show up as dark marks. Healthy larvae react to the touch by vIgorous movement and attempts to bite.
Reject any larvae, which are shrunken or aged which have recently moulted. or which are In a pre. pupal stage.
Weigh each larva and place It In a glass receptacle marking the receptacle with the weight of the larva. Make up two groups with the weight ranges:
Larvae with a mass larger than 150mg in mass are unsuitable as they can pupate during the course of the test.
5.1 BIological material
5.1.1 HyloIrupes bajulus (Llnnaeus) larvae
5.1.2 Source of larvae
The larvae shall preferably be obtained from cultures reared according to the method described in Annex B.
Larvae can also be taken from naturally infested wood. in which case they should be transferred into sapwood of pine and stored for at least four weeks under the rearing conditions specified in Annex B.
5.1.3 Provision of larvae
Carefully split or crumble InEestt!d blocks to extract larvae.
Keep the larvae separate from one another in glass receptacles for two or three days in the culturing chamber (5.3.1) to check they are healthy.
5.1.4 ChoIce of larvae
Use only healthy larvae in the test.
NOTE A healthy larva can be recognized by Ivoly-white colour. Its firm consistency and rounded appearance, and by the absence of wounds or bites. whrch show up as dark marks. Healthy larvae react to the touch by vIgorous movement and attempts to bite.
Reject any larvae, which are shrunken or aged which have recently moulted. or which are In a pre. pupal stage.
Weigh each larva and place It In a glass receptacle marking the receptacle with the weight of the larva. Make up two groups with the weight ranges:
Larvae with a mass larger than 150mg in mass are unsuitable as they can pupate during the course of the test.
